Volume 6, Issue 4, July 2018, Page: 99-102
Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM
Junwei Liu, High-throughput Molecular Drug Discovery Center, Tianjin International Joint Academy of Biomedicine, Tianjin International Joint Academy of Biomedicine Co., Ltd, Tianjin, People’s Republic of China; Xu He (Tianjin) Medical Technology Co., Ltd, Tianjin, People’s Republic of China
Jiaxing Wang, College of Pharmacy, Nankai University, Tianjin, People’s Republic of China
Jinfeng Wang, College of Pharmacy, Nankai University, Tianjin, People’s Republic of China
Li Liu, College of Pharmacy, Nankai University, Tianjin, People’s Republic of China
Jin Li, College of Pharmacy, Nankai University, Tianjin, People’s Republic of China
Haiqiu Ma, High-throughput Molecular Drug Discovery Center, Tianjin International Joint Academy of Biomedicine, Tianjin International Joint Academy of Biomedicine Co., Ltd, Tianjin, People’s Republic of China; Xu He (Tianjin) Medical Technology Co., Ltd, Tianjin, People’s Republic of China
Received: Oct. 17, 2018;       Published: Oct. 18, 2018
DOI: 10.11648/j.ajcem.20180604.13      View  248      Downloads  4
Abstract
Aim: The HFMD caused by EV71 virus has extremely high lethality and morbidity. EV71's pathogenesis has not understood thoroughly and the antiviral drug has been under development, which led to its emergence as a clinically fatal neurotropic virus. Early diagnosis is one of the main methods to reduce mortality. The aim of this study was to establish and evaluate a colloidal gold immunochromatography for qualitatively detecting the EV71-IgM in serum sample. Method: In this study, the different kinds of coating antigen were the focus of optimization. The pH value, particle size of the colloidal gold and other parameters and materials were also considered to achieve the good performance in detecting strips. The CA16-positive, EV71-positive and negative serum were used to compare and evaluate the accuracy and specificity of strips. Result: The accuracy of the strips coated by EV71-VP1-VP2-VP3 was higher than the EV71-VP1 strips 20%. The specificity of the EV71-VP1 strips was slightly higher than EV71-VP1-VP2-VP3 strips according to CA16 positive serum samples. Compared with commercial strips, this diagnostic method just needed 2 µL serum samples for detection, and the accuracy of the EV71-VP1-VP2-VP3 strips was significantly higher than the commercial strips. Conclusion: The developed method established in this study can apply to early diagnosis of HFMD caused by EV71 virus.
Keywords
HFMD, EV71-IgM, Colloidal Gold
To cite this article
Junwei Liu, Jiaxing Wang, Jinfeng Wang, Li Liu, Jin Li, Haiqiu Ma, Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM, American Journal of Clinical and Experimental Medicine. Vol. 6, No. 4, 2018, pp. 99-102. doi: 10.11648/j.ajcem.20180604.13
Reference
[1]
Tan E L, Chow V T, Quak S H, et al. Development of multiplex real-time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16. Diagn Micr Infec Dis 2008; 61: 294-301.
[2]
Kan X W, Justin J C. Antiviral screen identifies EV71 inhibitors and reveals camptothecin-target, DNA topoisomerase 1 as a novel EV71 host factor. Antiviral Research 2017; 143: 122-133.
[3]
Ma H Y, Lu C Y, Tsao K C, et al. Association of EV71 3C polymorphisms with clinical severity. Journal of Microbiology, Immunology and Infection 2017; 28: 1-6.
[4]
Peter C. McMinn. An overview of the evolution of enterovirus 71 and its clinical and public health significance. Fems Microbiology Rev 2003; 26: 91-107.
[5]
Li H G, Lao Q. The pulmonary complications associated with EV71-infected hand–foot–mouth disease. Radiology of Infectious Diseases 2017; 4(4): 137-142.
[6]
King A M Q, Brown F, Christian P, et al. 2000. Picornaviridae. In: Virus Taxonomy, Van Regenmortel M H V, Fauquet C M, Bishop D H L. et al., editors. Seventh Report of the International Committee for the Taxonomy of Viruses. New York: Academic Press. p 657-673.
[7]
Schmidt N J, Lennette E H, Ho H H. An apparently new enterovirus isolated from patients with disease of the central nervous system. J Infect Dis 1974; 129: 304-309.
[8]
Xing W, Liao Q, Viboud C, et al. Hand, foot, and mouth disease in China, 2008-12: an epidemiological study. Lancet Infect Dis 2014; 14(4): 308-318.
[9]
Chia M Y, Chiang P S, Chung W Y, et al. Epidemiology of Enterovirus 71 Infections in Taiwan. Pediatrics & Neonatology 2014; 55 (4): 243-249.
[10]
Liu C C, Chang H W, Yang G, et al. Development of a quantitative enzyme linked immunosorbent assay for monitoring the Enterovirus 71 vaccine manufacturing process. Journal of Virological Methods. 2011; 176 (1-2): 60-68.
[11]
Liu C C, Guo M S. Wu S R, et al. Immunological and biochemical characterizations of coxsackievirus A6 and A10 viral particles. Antiviral Research. 2016; 129: 58-66.
[12]
Xu F H, He D L, He S Z, et al. Development of an IgM-capture ELISA for Coxsackievirus A16 infection. Journal of Virological Methods. 2011; 171(1): 107-110.
[13]
Hou Y H, Wang J J, Jiang Y Z, et al. A colorimetric and electrochemical immunosensor for point-of-care detection of enterovirus 71. Biosensors and Bioelectronics. 2018; 99: 186-192.
[14]
Sunita S, Chow V T K, Phoon M C, et al. Direct Detection of Enterovirus 71 (EV71) in Clinical Specimens from a Hand, Foot, and Mouth Disease Outbreak in Singapore by Reverse Transcription-PCR with Universal Enterovirus and EV71-Specific Primers. J. Clin. Microbiol. 2002; 40(8): 2823-2827.
[15]
Jiang B, Zhang J, You X, et al. Diagnosis of hand, foot, and mouth disease caused by EV71 and other enteroviruses by a one-step, single tube, duplex RT-PCR. J Med Virol. 2012; 84(11):1803-1808.
[16]
Jiang B, Zhang J, You X, et al. Diagnosis of hand, foot, and mouth diseas-e caused by EV71 and other enteroviruses by a one-step, single tube, dup-lex RT-PCR. J Med Virol. 2012; 84: 1803-1808.
[17]
Wang M, Jiang S, Wang Y. Recombinant VP1 protein expressed in Pichia pastoris induces protective immune responses against EV71 in mice. Biochem Biophys Res Commun. 2013; 430(1):387-393.
[18]
Huang X, Liu G, Hu X, et al. Expression and activity determination of recombinant capsid protein VP2 gene of enterovirus type 71. Zhonghua Yu Fang Yi Xue Za Zhi. 2014; 48(4):324-327.
[19]
Wang C B, You A P, Tian X G, et al. Analysis and solution of false-positives when testing CVA16 sera using an antibody assay against the EV71 virus. Virus Research. 2013; 176 (1-2):33-36.
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