Volume 7, Issue 5, September 2019, Page: 111-118
Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis
Fu Xianzhao, Clinic Medical College, Youjiang Medical National College, Baise, China
Li Chunyan, Clinic Medical College, Youjiang Medical National College, Baise, China
Huang Wenhua, Clinic Medical College, Youjiang Medical National College, Baise, China
Li Xingchan, Clinic Medical College, Youjiang Medical National College, Baise, China
Qiu Haixian, Clinic Medical College, Youjiang Medical National College, Baise, China
Huang Guangming, Clinic Medical College, Youjiang Medical National College, Baise, China
Cao Qiuxia, Clinic Medical College, Youjiang Medical National College, Baise, China
Lu Mian, Clinic Medical College, Youjiang Medical National College, Baise, China
Received: Sep. 19, 2019;       Accepted: Sep. 28, 2019;       Published: Oct. 15, 2019
DOI: 10.11648/j.ajcem.20190705.12      View  44      Downloads  15
Abstract
To explore the mechanism of Huoxue Jiedu Jiangtang Recipe (HJJR) in alleviating endoplasmic reticulum stress (ERS) - inflammation, inhibiting ERS apoptotic bypass of CHOP and alleviating diabetic atherosclerosis (AS). Diabetic AS rats were duplicated by feeding of high lipid diet and intraperitoneal injection of streptozotocin. After diabetes modeled successfully, the rats were randomly divided into model group, low-dose HJJR group (HJJR1), high-dose HJJR group (HJJR2) and western medicine group (Gliquidone+ Benazepril), and accepted corresponding drugs for 2 months respectively. The same batch of rats were taken as normal control group and fed with common diet. The levels of glycosylated hemoglobin (GHb), blood lipid, fasting serum insulin (FINS) and insulin resistance index (IRI) were measured in each group. The contents of inflammatory factors TNF-α and IL-6 were detected by immunohistochemistry. The mRNA transcription of GRP78 and caspase-12 were tested by reverse- transcription polymerase chain reaction (RT-PCR). The apoptotic level of aortic cells was checked by TUNEL. Compared with the model group, all drug groups could significantly reduce GHb, IRI, TG and LDL-C (P<0.05), increase FINS and HDL-C (P<0.05), down-regulate transcription of CHOP and GRP78 (P<0.05), reduce inflammatory factors TNF-a, IL-6, and decreased aortic apoptosis index (AI) (P<0.05). Compared with the western medicine group, the effect of HJJR2 group was more significant (P<0.05). HJJR could alleviate insulin resistance, correct lipid metabolism disorder, depress endoplasmic reticulum stress-induced inflammatory reaction, and inhibit endoplasmic reticulum stress-induced apoptotic bypass of CHOP in arterial cells. The therapeutic effect is dose dependent.
Keywords
Diabetes, Atherosclerosis, Endoplasmic Reticulum Stress, CCAAT Enhance-Binding Protein Homologous Protein, Huoxue Jiedu Jiangtang Recipe
To cite this article
Fu Xianzhao, Li Chunyan, Huang Wenhua, Li Xingchan, Qiu Haixian, Huang Guangming, Cao Qiuxia, Lu Mian, Effect of “Nourishing Yin and Qi, Promoting Blood Circulation and Detoxification” on Endoplasmic Reticulum Stress CHOP Apoptotic Bypass in Diabetic Atherosclerosis, American Journal of Clinical and Experimental Medicine. Vol. 7, No. 5, 2019, pp. 111-118. doi: 10.11648/j.ajcem.20190705.12
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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