Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors
Sekongo Yassongui Mamadou,
Dasse Sery Romuald,
Altemeyer Anaïs,
Soraya Amar,
Tayou Claude,
Anani Ludovic,
Kassogue Kadidia,
Geisen Cristof,
Herbrich Anne,
Kouamenan Sidonie,
Konate Seidou
Issue:
Volume 7, Issue 6, November 2019
Pages:
126-129
Received:
16 October 2019
Accepted:
6 November 2019
Published:
27 November 2019
Abstract: To conduct a study project on the inactivation of pathogens in whole blood using acridine derivatives in Africa, in order to prevent the transmission of pathogens, it is important to look for possible immunization to acridine in the blood donor population. It is therefore important to undertake an estimate of the prevalence in the sub-Saharan population in order to guide the design of the clinical study that will use the INTERCEPT Red Blood System procedure on whole blood for transfusion. to define the starting point in the evaluation of the immunological safety and transfusion safety of the product treated by this process. The objective of this study is to determine the prevalence of AAA among blood donors in sub-Saharan Africa. We conducted a multicenter prospective descriptive study of 902 blood donors collected in Côte d'Ivoire, Benin and Cameroon over the period from June 2015 to January 2017. Blood samples were collected from voluntary blood donors, of any sex, aged between 18 and 65, having given their consent for the study and having participated in the medical consultation for the donation of blood. The samples were analyzed according to the technique of the RAI gel card of the company BIORAD after centrifugation and incubation using test red cells treated with S-303 and glutathione. In the case of positive RAI results, to confirm the presence of anti-acridine, the donor plasma should be incubated with S-300. S-300 is a degradation product of S-303. The donor serum and S-300 are then incubated with the same red test cells. S-300 binds to the antibody and produces a negative result in the gel map in the presence of anti-acridin antibodies. Of the 903 samples tested both at the Abidjan laboratory in Côte D'Ivoire and at the Frankfurt laboratory, we found 1 positive sample and 8 reactive samples (positive for anti-erythrocyte antibodies). Positive donor plasma was incubated with S-300 which is the degradation product of S-303. The result is always positive, whereas according to the instructions of the reference laboratory of Frankfurt, it should be negative in case of presence of Locustacan. The results on AAA testing among 903 donors in three sub-Saharan countries show the absence of AAA in the sample of subjects included in the study according to the hypothesis emitted from this study. This opens the door to the prospect of conducting a clinical study on the inactivation of pathogens by acridine derivatives.
Abstract: To conduct a study project on the inactivation of pathogens in whole blood using acridine derivatives in Africa, in order to prevent the transmission of pathogens, it is important to look for possible immunization to acridine in the blood donor population. It is therefore important to undertake an estimate of the prevalence in the sub-Saharan popul...
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Evaluation of the Performance of an Automated Chemiluminescent Method (Access Beckman Coulter) for Determination of Salivary Cortisol and Clinical Utility
Maria Alejandra Kloberdanz,
Lucia Fernandez,
Agustina Peverini,
Fernando Smithuis,
Adrian Aymard,
Claudio Aranda,
Bibiana Fabre,
Martin Repetto,
Adriana Oneto
Issue:
Volume 7, Issue 6, November 2019
Pages:
130-134
Received:
29 October 2019
Accepted:
20 November 2019
Published:
6 December 2019
Abstract: The diagnostic procedure for the evaluation of Cushing’s syndrome is performed by any of the following biochemical tests: urine free cortisol, salivary cortisol at 11 pm and serum cortisol post 1 mg of dexamethasone. Collection of saliva samples is simple and noninvasive, thus being a method of choice for the evaluation of risk populations. The aim of this work is to analyze the performance of an automated chemiluminescent method for measurement of salivary cortisol at 11 pm according to the new quality guidelines and assess its clinical utility. Cortisol levels were measured in samples obtained by passive drooling from 32 healthy subjects and 9 patients with Cushing’s syndrome. Matrix effect, linearity, limit of blank, limit of quantitation, recovery and diagnostic performance were assessed. The Unicel 600 DXI Access Beckman Coulter chemiluminescent automated analyzer was used. The standard curve provided by the manufacturer was adapted to measure cortisol concentrations in saliva. Matrix effect: equation of the curve using salivary matrix: y=-1.824x+3.491 (95% CI=-2.068 to -1.582) vs. Equation of the curve using diluent matrix: y=-1.833x+3.394 (95% CI=-1.961 to -1.704). There is overlapping of both curves. Linearity: linear assay between 1.8 nmol/L and 108.0 nmol/L. Limit of blank: 0.1 nmol/L. Limit of quantitation: 1.8 nmol/L (TAE of 25%). Recovery: standard cortisol solution concentration 5 nmol/L: 102%; 10 nmol/L: 107%; 40nmol/L: 115%. Diagnostic performance: median and ranges in healthy subjects: 2.0 nmol/L (<2.0-9.0 nmol/L); Cushing’s syndrome: 30.3 nmol/L (15.4-61.0 nmol/L). ROC curve cutoff value: 9.0 nmol/L (100% Specificity; 100% Sensitivity; AUC=1.00). The method used provides excellent analytical performance for cortisol measurement in saliva at 11 pm, which makes it a valuable biochemical tool both for screening populations at risk for Cushing’s syndrome and for the follow-up and diagnosis of this condition.
Abstract: The diagnostic procedure for the evaluation of Cushing’s syndrome is performed by any of the following biochemical tests: urine free cortisol, salivary cortisol at 11 pm and serum cortisol post 1 mg of dexamethasone. Collection of saliva samples is simple and noninvasive, thus being a method of choice for the evaluation of risk populations. The aim...
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Idea on Holistic Medicine Based on the Electron Leak Pathways of Mitochondrial Respiratory Chain
Issue:
Volume 7, Issue 6, November 2019
Pages:
135-142
Received:
18 September 2019
Accepted:
21 October 2019
Published:
13 December 2019
Abstract: With the development of physical technology, life science has reached the molecular level. With the development of molecular biology, western medicine has carried out in-depth analysis and Experimental Research on diseases. Nevertheless, wise thinking about the theory and current situation of Western medicine can still find some problems worth to investigation. With the breakthroughs in cell and molecular biology, protein function and gene regulation have become the starting point and theoretical basis for thinking about medical problems and developing drugs. However, the human body is a multicellular organism, which is the coordination of multiple functional organs formed by orderly accumulation of cells. At present, the knowledge of molecular biology on the orderly accumulation of cells is still scarce in life science. This knowledge black hole makes Western medicine far from being based on a comprehensive understanding on the human body, thus making the scientific nature of Western medicine imperfect. Mitochondrial symbiosis is a landmark event in which life evolves from a single cell to a multicellular one. In our lab the discovery of electron leak pathways of the mitochondrial respiratory chain reminds me to describe the function of respiratory chain as the molecular "life-engine". The role of ROS generated in mitochondria through the electron leak pathways of the respiratory chain has become a new investigative field of health and longevity. The signal effect of ROS on antioxidant activity and tissue repair activity has become a hotspot in medical research. An idea to explore medical problems in perspective of bioenergetics came into being in my mind. In 2016, I published a paper in the Journal of clinical and experimental cardiology, which suggested that the human body should be regarded as a whole composed of a huge number of "life engines", and human health and medicine should be studied with thermodynamics. A big question is that the ROS generated by the electron leakage of respiratory chain has two opposite roles: it causes aging and diseases by the oxygen toxicity of ROS and it role as signal to stimulate antioxidative activity or tissue repair ability. Then how the human body makes decision to tell ROS play as a positive or negative role? Answering this question takes me long time searching. In this paper, I will elaborate on this issue from the perspective of cosmic energetics.
Abstract: With the development of physical technology, life science has reached the molecular level. With the development of molecular biology, western medicine has carried out in-depth analysis and Experimental Research on diseases. Nevertheless, wise thinking about the theory and current situation of Western medicine can still find some problems worth to i...
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